TABLE 1.

The secondary structure contents (SSC) estimated from the CD spectra of native and dialysis-renatured MfpA

Fractionsa	NAAb(x-ray)	Native MfpA				Dialysis-renatured MfpA
		Fractionc	RMSDd	NRMSDd	NAAb(CD)	Fraction	RMSD	NRMSD	NAA (CD)
Htotal	16	0.035	0.033	0.681	1–12	0.152	0.039	0.249	21–35
Stotal	40	0.188	0.096	0.454	17–52	0.317	0.008	0.027	57–59
Turns	40	0.221	0.026	0.118	36–45	0.216	0.011	0.049	38–42
Unordered	87	0.574	0.179	0.298	72–138	0.312	0.039	0.125	50–64
Overalle			0.104	0.307			0.029	0.11

^aSecondary structure types: H_total, total helixes, S_total, total strands, Turns, turns; Unordered, the remainder of the protein

^bNumber of amino acids included in the given type the secondary structure calculated from DSSP assignment of the crystal structure, N_AA (x-ray) or from the CD spectra of MfpA, N_AA (CD)

^cCalculated fraction of each secondary structure type

^dThe values of r.m.s.d. and n.r.m.s.d. in this table reflect the analysis of the CD spectrum by the different programs and protein reference sets as described under “Experimental Procedures”

^eThe overall performance of the CD analysis determined by considering all secondary structures collectively