FIGURE 9.

p53 dependence of TLE1 and mSin3A occupancy at the ADF element in the liver chromatin and p53/TLE1 interaction in liver nuclear extract. A, sequential ChIP using anti-p53 antibody for the first step ChIP and antibodies to p53, TLE1, and mSin3A for the second step ChIP. Analysis was performed on individual liver samples from two young (4-month-old) rats and two old (22-month-old) rats. The bar graphs represent average values (±S.D.) from q-PCR results (conducted in duplicate) derived from liver samples of two individual animals from each age group. Representative semiquantitative PCR results for one young and one old animal are shown below. Signals from the liver chromatin of young animals were at the background level (or non-existent) when chromatin fragments were immunoprecipitated with anti-p53 antibody at the first step ChIP. B, co-IP of p53 and TLE1 from RLNE. WB, Western blot.