Figure 10. Histopathology in other tissues.

(A) S1PL^+/+ heart: There is minimal interstitial tissue in Wt myocardium. (B) S1PL^−/− heart: There is a patchy to diffuse expansion of the interstitium by numerous variably-sized vacuoles and vacuolated mesenchymal cells that separate the cardiomyocytes. Scattered cardiomyocytes contain indistinct foamy inclusions and sharply demarcated clear vacuoles. (C) S1PL^+/+ urinary bladder: Normal urothelium lines the urinary bladder in Wt mice (D) S1PL^−/− urinary bladder: There is widespread ballooning vacuolization, degeneration, and apoptosis of urothelial cell. Lesions are more severe in the superficial umbrella cell layer, where degenerating cells are interspersed among vacuolated urothelial cells. (E) Bone, aged S1PL^H/− mouse: Small numbers of distended osteoclasts are present multifocally at the growth plate only. No increase in trabecular bone was evident. (F) Urinary bladder, aged S1PL^H/− mouse: Rare vacuolated urothelial cells were present in the superficial mucosa. H&E stain.