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. 2009 Jan 1;4(1):e4104. doi: 10.1371/journal.pone.0004104

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Takahashi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Western blot shows that PQBP1-shRNA-IRES-ZsGreen plasmid suppresses PQBP1 protein in primary mouse white adipocytes. Non-silencing shRNA (ns-ShRNA) was used as a control. The band intensities are corrected with GAPDH and standardized with non-transfected cells (NT). B) Primary white adipocytes were stained by Oil Red after transfection with PQBP1-shRNA-ZsGreen or non-silencing shRNA-ZsGreen plasmid. Non-transfected cells (arrowheads) possess large lipid droplets while PQBP1-shRNA-transfected cells (green) show small granules (arrows). Non-silencing shRNA-transfection did not affect the size of lipid granules (arrows in lower panels).