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. 2008 Dec 31;3(12):e4106. doi: 10.1371/journal.pone.0004106

Figure 6. HongrES1 knockdown enlarged the action of BSA on sperm in vitro.

Figure 6

(A) The change of protein tyrosine phosphorylation on siRNA-treated sperm after incubation in the presence or absence of BSA. The spermatozoa of caudal epididymis from rats treated by siRNAs for 48 h were collected and incubated. The spermatozoa total proteins were collected at 0 h, 1 h and 3 h of incubation; α-tubulin was used as loading control. Csi, scrambled siRNA control; Hsi2, the siRNA specifically targeting the site of HongrES1 sequence. (B) Flow cytometric dot-plots of merocyanine and Yo-Pro-1 stained sperm population. The spermatozoa of caudal epididymis from rats treated by siRNAs for 48 h were collected and incubated. The spermatozoa were stained and subjected to flow cytometry as described in Material and Methods. D, dead cells (stained with Yo-Pro-1); L, live cells showing merocyanine staining. Csi, scrambled siRNA control; Hsi1 and Hsi2, two siRNAs specifically targeting the different sites of HongrES1 sequence.