. Author manuscript; available in PMC: 2009 Sep 15.

Published in final edited form as: Bioorg Med Chem Lett. 2008 Aug 6;18(18):5041–5045. doi: 10.1016/j.bmcl.2008.08.006

Table 2.

Inhibition of tubulin polymerization and colchicine binding by compounds 2a, 4f-g, 5, and CA-4

Compound	Tubulin assembly^a IC₅₀ ± SD (μM)	Colchicine binding^b ±SD (%)
Compound	Tubulin assembly^a IC₅₀ ± SD (μM)	1 μM inhibitor	5 μM inhibitor
n.d., not done.
2a	1.9 ± 0.1	25 ± 2	71 ± 1
4f	0.6 ± 0.02	71 ± 0.6	84 ± 3
4g	0.6 ± 0.03	67 ± 2	82 ± 2
5	5.4 ± 0.4	n.d.	27 ± 0.1
CA-4 (1)	1.2 ± 0.1	90 ± 1	99 ± 0.7

Inhibition of tubulin polymerization. Tubulin was at 10 μM.

Inhibition of [³H]colchicine binding. Tubulin and colchicine were at 1 and 5 μM, respectively, and the tested compound was at the indicated concentration.