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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Res Microbiol. 2008 Jul 31;159(7-8):562–568. doi: 10.1016/j.resmic.2008.07.003

Fig. 1.

Fig. 1

Adenylate cyclase is required for swimming motility and flagellum production by S. marcescens. A. Photograph of swimming zones through 0.3% agar by the cyaA (adenylate cyclase) mutant strain with either an empty vector or the wild-type cyaA gene under control of the Plac promoter on a multicopy plasmid (pcyaA). B–C. TEM micrographs of wild-type (B) and cyaA mutant (C) cells. The black arrow denotes flagella and the gray arrows indicate fimbriae. The bar indicates either 500 nm (B) or 100 nm (C). D. PAGE analysis of surface protein fractions from stationary phase wild-type and cyaA cells with vector control or pcyaA. The flagellin protein was verified by mass spectroscopy.