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. Author manuscript; available in PMC: 2008 Dec 22.
Published in final edited form as: J Biol Chem. 2000 Dec 15;276(11):8616–8622. doi: 10.1074/jbc.M009901200

Fig. 2. Analysis of sc-mtRNA polymerase amino-terminal domain mutations.

Fig. 2

A, growth phenotypes. Shown to the left is the RPO41 allele expressed in each strain after plasmid shuffle in GS112. At the top of each column of panels, the growth medium (YPG or YPD) and growth temperature (30 or 37 °C) are indicated. For example, the strain that is expressing wild-type allele (RPO41) after plasmid shuffle is indicated. Serial 10-fold dilutions are plated from left to right within each panel and, in each row of panels, identical cultures were plated. B, western analysis of rpo41 point-mutant strains after plasmid shuffle. The blot was first probed with an anti-RPO41p antibody and the location of the 150-kDa, full-length mtRNA polymerase (Rpo41p) is indicated. The RPO41 null and point-mutant strains analyzed are indicated above each lane. The identical blot was stripped and probed again with an antibody against Yrb1p (yeast Ran-binding protein, a nuclear transport protein) to serve as a control for the amount of protein loaded in each lane.