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. Author manuscript; available in PMC: 2008 Dec 22.
Published in final edited form as: J Biol Chem. 2003 Mar 10;278(20):18695–18701. doi: 10.1074/jbc.M301399200

Fig. 4. Sls1p is a component of the mitochondrial nucleoid.

Fig. 4

Mitochondrial lysates and nucleoid fractions were prepared as described under “Experimental Procedures.” V5-tagged Sls1p was detected by Western analysis using an anti-V5 antibody (top panel). A separate gel was run and silver-stained to visualize the amount of protein loaded (bottom panel). Lanes 1–4 are the mitochondrial lysates prior to fractionation, and lanes 5–8 are the purified nucleoid fractions. The sample analyzed is indicated at the top of each lane: MSR107 (wt); MSR107 treated with DNase I (wt +DNase); MSR110(Δ3) and MSR107 without the energy regenerating system (wt −energy).