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. Author manuscript; available in PMC: 2008 Dec 22.
Published in final edited form as: J Biol Chem. 2003 Mar 10;278(20):18695–18701. doi: 10.1074/jbc.M301399200

Fig. 5. Overexpression of Sls1p increases the steady-state levels of mtDNA-encoded proteins in ATD mutant strains.

Fig. 5

Western analysis of ATD mutants with and without a plasmid that overexpresses Sls1p. Blots of total mitochondrial protein (40 μg/lane) were sequentially probed with antibodies to mtDNA-encoded Cox2p, Cox1p, and the nucleus-encoded porin. The mutation in the RPO41 gene harbored by each strain tested is indicated at the top of the figure in the same manner as in Fig. 2A. The 1st to 5th lanes are the strains without the SLS1 plasmid as a control, and the 6th to the 10th lanes (bracketed) are the strains expressing SLS1 from pRMS5-6 (9), indicated as (+SLS1).