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. 2008 Dec 24;3(12):e4032. doi: 10.1371/journal.pone.0004032

Figure 1. Effect of hPc2 on sumoylation of human CBS.

Figure 1

A. The in vitro sumoylation reaction was performed as described under Methods and SUMO-1 antibody was used to detect the presence of sumoylated CBS. Lanes 1 and 2 show sumoylation reactions that were conducted in the absence (lane 1) and presence (lane 2) of hPc2. B and C. Equal loading controls for CBS in the two reaction mixtures. The CBS concentration prior to the start of the sumoylation reaction was identical in both lanes as detected by Coomassie blue staining (B) or by Western blot analysis using CBS antibody (C).