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. 2009 Jan 5;4(1):e4118. doi: 10.1371/journal.pone.0004118

Table 1. Protocol outline for the siRNA transfection/infection experiments in mice.

group concentration/combination of siRNA's (pmol) time of siRNA application Transfection reagent Infection
A sigB3 (187.5) 24 h before Ia lipofectamine Ab4
B sigB3 (187.5) 0.5 h before I lipofectamine Ab4
C siOri2 (187.5) 24 h before I lipofectamine Ab4
D siOri2 (187.5) 0.5 h before I lipofectamine Ab4
E sigB3/siOri2 (31.25/31.25) 24 h before I lipofectamine Ab4
F sigB3/siOri2 (31.25/31.25) 0.5 h before I lipofectamine Ab4
G sigB3/siOri2 (62.5/62.5) 24 h before I lipofectamine Ab4
H sigB3/siOri2 (62.5/62.5) 0.5 h before I lipofectamine Ab4
I siLuc (250) 24 h before I lipofectamine Ab4
J no siRNA 24 h before I lipofectamine Ab4
K siLuc (250) 12 h before I lipofectamine Ab4
L siLuc (250) 12 h before I PBS Ab4
M sigB3/siOri2 (62.5/62.5) 12 h before I lipofectamine Ab4
N sigB3/siOri2 (62.5/62.5) 6 h before I lipofectamine Ab4
O sigB3/siOri2 (62.5/62.5) 12 h before I PBS Ab4
P sigB3/siOri2 (62.5/62.5) 6 h before I PBS Ab4
Q sigB3/siOri2 (62.5/62.5) 1 h after I PBS Ab4
R sigB3/siOri2 (62.5/62.5) 6 h after I PBS Ab4
S sigB3/siOri2 (62.5/62.5) 12 h after I PBS Ab4
T sigB3/siOri2 (62.5/62.5) 24 h after I PBS Ab4
control b no siRNA (PBS) 24 h before medium medium
control no siRNA (PBS) 12 h before medium Medium
a

I: infection.

b

control: to evaluate weight loss due to anesthesia.