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| Real time PCR | ||
| Gene | Primers (5′→3′) a | MGB probe |
| EHV-1 IR6 | F: GCGAAGTACCCCTCGTTCATCT | TCGCGACACCGCCT |
| R: ATGCTCGGGCGCTCCTACT | ||
| EHV-1 gB | F: CGCTGAGGATGGAGACTTTTACA | CCACCGCCTACCGGATCCACC |
| R: GGTGGTTCGATGCGTACG | ||
| EHV-1 Ori | F: TGGTAACGGTGGGCCTTAGT | TTGATACGGCTCATT TCCACAGC |
| R: GGGCTTGACGTAGGCAAACA | ||
| rabbit β-actin | F: CGAGATCGTGCGGGACAT | AAGGAGAAGCTGTGCTACGTGGCGCT |
| R: GCCATCTCCTGCTCGAAGTC | ||
F: Forward primer, R: Reverse primer.
