Table 1. Phosphopeptides derived from sites of tyrosine phosphorylation on ErbB4.
All peptides were labeled on their N-terminus with 5(6)-TAMRA (denoted ‘d’) and contained a C-terminal Asp residue. The red ‘pY’ denotes phosphotyrosine.
| pTyr site | Peptide sequence |
|---|---|
| ErbB4-875 | dRLLEGDEKEpYNADGGKMD |
| ErbB4-1035 | dQAFNIPPPIpYTSRARIDD |
| ErbB4-1056 | dEIGHSPPPApYTPMSGNQD |
| ErbB4-1066 | dTPMSGNQFVpYRDGGFAAD |
| ErbB4-1081 | dAAEQGVSVPpYRAPTSTID |
| ErbB4-1128 | dVQEDSSTQRpYSADPTVFD |
| ErbB4-1150 | dPRGELDEEGpYMTPMRDKD |
| ErbB4-1162 | dPMRDKPKQEpYLNPVEEND |
| ErbB4-1188 | dDLQALDNPEpYHNASNGPD |
| ErbB4-1202 | dNGPPKAEDEpYVNEPLYLD |
| ErbB4-1208 | dEDEYVNEPLpYLNTFANTD |
| ErbB4-1221 | dFANTLGKAEpYLKNNILSD |
| ErbB4-1242 | dAKKAFDNPDpYWNHSLPPD |
| ErbB4-1258 | dPRSTLQHPDpYLQEYSTKD |
| ErbB4-1262 * | dLQHPDYLQEpYSTKYFYKD |
| ErbB4-1266 * | dDYLQEYSTKpYFYKQNGRD |
| ErbB4-1268 | dLQEYSTKYFpYKQNGRIRD |
| ErbB4-1284 § | dRPIVAENPEpYLSEFSLKD |
| ErbB4-1301 | dPGTVLPPPPpYRHRNTVVD |
No product of the correct molecular weight was obtained for these peptides.
This peptide exhibited high levels of background binding on the protein microarrays, precluding an analysis of its interactions.