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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: Exp Cell Res. 2008 Aug 8;314(17):3107–3117. doi: 10.1016/j.yexcr.2008.07.028

Figure 4.

Figure 4

Recombinant GST tagged HDGF was produced. This figure shows Coomassie blue staining of the purified GST-tagged HDGF (1 μg) cleaved by thrombin (A lane 1), purified recombinant HDGF (0.2 μg) after removal of GST peptide (A lane 2) and purified recombinant protein (0.2 μg) analyzed by Western blotting using the antibodies to the proteins. (A lane 3). Recombinant HDGF was able to induce MSC migration in a concentration-dependent manner from 0.4 to 100 ng/ml. Migration of MSCs in response to the medium with 2% FBS (control) is shown. Boiled, recombinant HDGF did not induce MSC migration and addition of an anti-HDGF antibody at a concentration of 12 μg/ml blocks migration in response to 100ng/mL HDGF comparisons: *, significantly higher than control, p= 0.01. (B). An anti-HDGF antibody also blocks the migration of MSCs in response to MDA-MB-231 conditioned medium (C). The anti-HDGF antibody was added to CM at concentrations from 1 to 12 μg/ml and a concentration-dependent decrease in MSC migration was observed. Antibody denatured by boiling did not affect MSC migration. Statistical significance *, significantly lower than CM, p= 0.002.