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. Author manuscript; available in PMC: 2008 Dec 22.
Published in final edited form as: Ann Biomed Eng. 2008 Oct 7;36(12):2134–2148. doi: 10.1007/s10439-008-9575-z

FIGURE 4.

FIGURE 4

Effect of competitors on receptor binding. SMC Binding studies at 4 °C with 125I-FGF-2 (a), 125I-HB-EGF (b) and 125I-EGF (c) in the presence of FGF-2 (□), HB-EGF (○) and EGF (●) were performed as described in “Methods” section. Radiolabeled growth factor bound is plotted scaled to the amount of radiolabeled growth factor found in the absence of any competing unlabeled growth factor. All radiolabeled growth factors were used at a concentration of 5 ng/mL or 0.28 nM 125I-FGF-2 (a), 0.42 nM 125I-HB-EGF (b), or 0.83 nM 125I-EGF (c). Measurements (mean ± SE, n = 3) are representative of at least three independent experiments. *Significantly (p < 0.05) different from binding with no competitor.