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Effect of HB-EGF affinity for common site HSPG with FGF-2 added (simulations)—HB-EGF (0.5 ng/mL or 0.042 nM) bound to EGFR after 2.5 h incubation at 4 °C with FGF-2 (0 nM (○), 10 nM (●), 100 nM (◆), and 1000 nM (■) for “non-receptor-coupling” (a) or “receptor-coupling” (b) with varying affinity of HB-EGF for HSPG common sites. All other parameters as listed in Table 2.
