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Effect of FGF on EGF binding in the presence of HB-EGF. EGF (1 ng/mL or 0.16 nM) bound to EGFR after 2.5 h incubation at 4 °C with (●, ■) and without (○, □) HB-EGF (1 ng/mL or 0.083 nM) for “non-receptor-coupling” (□, ■) and “receptor-coupling” (○,●) models. All other parameters as listed in Table 2. Note direct overlap of binding of EGF in the absence of HB-EGF for both models (□,○).
