Figure 2.

Density of microvessels and hypoxia in tumor tissues. (A) Tumors from the RBP-J^-/- and control mice were sectioned at 10 µm and were stained for CD31 and HIF1α by immunohistochemistry. (B) Microvessels in (A; upper panel) were counted under a microscope, and microvessel densities (microvessels per field) were compared. (C) HIF1α-positive signals in (A; lower panel) were quantified using Image-Pro Plus 5.1 and were compared for statistical significance. Bars, means ± SD. *P < .05, **P < .01; n = 5.