Figure 6.

Histopathology of lung tumors that received bevacizumab therapy. (A) Histologic images of sections (3 µm) from untreated and bevacizumab-treated lung tumor sections that were stained with hematoxylin and eosin (H&E; the black dashed lines indicate areas of hemorrhage in the tumor), with anti-vWF (red arrows in A indicate vWF-positive vessels) and anti-PCNA (black arrows in A indicate PCNA-positive cells), respectively. Quantification of vWF-positive tumor vessels (B) and PCNA-positive tumor cells (C) by counting positively stained cells in ten randomly selected microscopic fields in seven mice is given. (D) Lung and lung tumor tissue density with or without Microfil were measured with micro-CT (60 areas were selected). Means ± SEM are shown. Scale bars, 40 µm.