Skip to main content
. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Neuropharmacology. 2008 Jun 27;55(4):500–508. doi: 10.1016/j.neuropharm.2008.06.039

Figure 3. Downregulation of mGlu1 expression in rat cerebellar neurons improves neuronal survival.

Figure 3

a, Toxicity measured 24 hrs after changing the medium from K25+B27 to K5+B27 is not prevented by either CPCCOEt (100 µM) or by MPEP (10 µM). b, Application of mGlu1-specific siRNA but not nonspecific siRNA (NS) to rat cerebellar neurons at the time of medium change from K25+B27 to K5+B27 attenuates toxicity induced by K5 conditions. c, mGlu1 siRNA abolishes ACPD (100 µM)-induced, CPCCOEt (100 µM)-sensitive, PI hydrolysis in rat cerebellar neurons measured 72 h after medium change from K25+B27 to K5+B27. Application of mGlu1-specific siRNA also attenuates enhanced mGlu1 expression as shown after 72 hrs by a representative Western blot (d), and its quantification using normalization with β-actin (e). Data are means with error bars representing S.E.M. from three to four separate experiments. *p<0.05 and **p<0.001 as compared to untreated K5 controls using Student’s t-test.