Table 4.

Mutant phenotyping experiments: growth media and experimental results for genes included in iAbaylyi^v3

Source of1		Essentiality		Specific metabolic pathways a priori involved
Carbon	nitrogen	E	D
acetate	ammonia	5	431	Glyoxylate shunt
L-asparagine	ammonia	3	445	Asparagine and aspartate degradation
D-2,3-butanediol	ammonia	10	433	Butanediol to acetoin to acetyl-coa degradation, glyoxylate shunt
D-glucarate	ammonia	5	413	Glucarate to 2-oxoglutarate degradation
β-D-glucose	ammonia	7	432	Entner-Doudoroff pathway
L-lactate	ammonia	2	445	Lactate dehydrogenase
quinate	ammonia	8	436	Quinate to protocatechuate to acetyl-coa and succinyl-coa degradation
succinate	urea	3	442	Urease

¹ Italic text indicates the changed carbon or nitrogen source with respect to the medium used for mutant construction (succinate and ammonia).

E: number of conditionally essential genes

D: number of dispensable genes