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. 2009 Jan;58(1):125–133. doi: 10.2337/db08-0400

FIG. 1.

FIG. 1.

The hepatic OPN and CD44 expression is significantly correlated with the grade of steatosis in morbidly obese patients. A: Hepatic OPN and CD44 mRNA expression levels were analyzed by real-time quantitative PCR in lean patients (controls; n = 6), in morbidly obese patients without liver steatosis (S0; n = 6), and in morbidly obese patients with S1 (n = 13), S2 (n = 9), and S3 (n = 24) steatosis. The gene expression of OPN and CD44 was normalized to the mRNA levels of RPLP0. Results are expressed relative to the expression level in controls and expressed as means ± SE. *P < 0.05 compared with controls; #P < 0.05 compared with S0; $P < 0.05 compared with S1. B: Total lysates from liver biopsies obtained from three morbidly obese patients without steatosis (S0) and six with severe steatosis (S3) were separated by SDS-PAGE and immunoblotted with anti-OPN or anti-GAPDH antibodies. A representative immunoblot is shown. C: Correlation between OPN and TNF-α mRNA expression levels (ΔCt) in lean subjects (n = 5) and morbidly obese patients without (n = 6) or with severe steatosis (n = 14) was analyzed using Spearman's rank correlation test.