FIG. 6.

In vivo cytotoxicity activity of alloreactive CD8 T-cells in mice treated with costimulation blockade. A: NOD and C57BL/6 male mice 6–8 weeks of age were untreated or injected with 1.0 mg anti-CD122 mAb (clone TMβ1). Twenty-four hours later, all groups were injected with 100 μg poly I:C, and 20 h later, spleen cells were recovered for analyses of in vitro NK cell cytotoxicity activity on the NK-sensitive cell line YAC-1, as previously described (25). B: NOD, C57BL/6, and NOD.B6 Idd3 mice were treated with costimulation blockade, depleted of NK cells and injected with CFSE-labeled spleen cells for an in vivo cytotoxicity assay, as described in research design and methods. ○, DST; ▾, DST + anti-CD154. The number of mice tested in each group is indicated below each strain tested. NOD vs. NOD.B6 Idd3, P < 0.005; C57BL/6 vs. NOD, P < 0.01 C57BL/6 vs. NOD.B6 Idd3, NS.