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. 2009 Jan;58(1):174–184. doi: 10.2337/db08-0862

FIG. 5.

FIG. 5.

Effect of insulin on calcium entry in MIN6 cells. A and B: Measurement of changes in [Ca2+]c. Fura-2–loaded MIN6 cells were incubated for 30 min in calcium-free KRB in the presence (B) and absence (A) of 10 nmol/l insulin. Extracellular medium was then switched to 2 mmol/l calcium-containing KRB with (○) or without (•) 75 μmol/l tranilast as indicated, and changes in the [Ca2+]c were monitored. The results are representative of at least 10 experiments. C: Measurement of calcium entry by monitoring Mn2+ quenching. Fura-2–loaded MIN6 cells were incubated for 20 min in the presence (•) or absence (○) of 10 nmol/l insulin. MnCl2 (0.1 mmol/l) was then added, and changes in the fluorescence were monitored. Values are the mean ± SE for five determinations and representative of five experiments with similar results. D: Effect of tranilast on Mn2+ quenching. Experiments were done as shown in C except that 75 μmol/l tranilast was added together with MnCl2.