TABLE 2.
List of proteins identified in conditioned media of primary human myotubes
| SwissProt | Peptides | Secreted | Evidence | |
|---|---|---|---|---|
| Immune response/complement | ||||
| Complement component 4B | P0C0L5 | 7 | Yes | 8,24 |
| Complement factor H-related 5 | Q9BXR6 | 2 | Yes | 8 |
| Growth factors/chemokines | ||||
| Epidermal growth factor | P01133 | 2 | Yes | Swiss-Prot |
| Fibroblast growth factor 17 | O60258 | 2 | Yes | Swiss-Prot |
| Insulin-like growth factor 1 | Q59GC5 | 1 | Yes | 24 |
| Interferon α2/β1 | Q14608 | 1 | Yes | Swiss-Prot |
| Lymphotactin | P47992 | 1 | Yes | Swiss-Prot |
| Monocyte chemotactic protein 4, MCP-4 | Q99616 | 2 | Yes | Swiss-Prot |
| Myostatin | Q8HY52 | 3 | Yes | Swiss-Prot |
| Cell adhesion/extracellular matrix | ||||
| Collagen, type III, α1 | P02461 | 2 | Yes | 17 |
| Collagen, type IV, α2 | P08572 | 4 | Yes | 17,24 |
| Collagen, type IV, α3 | Q01955 | 2 | Yes | 17,24 |
| Collagen, type I, α1 | P02452 | 5 | Yes | 8,17,23,24 |
| Extracellular matrix protein 2 | O94769 | 1 | Yes | 23,24 |
| Laminin, α2 | P24043 | 4 | Yes | 24 |
| Laminin, α3 | Q16787 | 3 | Yes | 24 |
| Tenascin C | P24821 | 5 | Yes | 24 |
| Tenascin XB | Q5JNX3 | 2 | Yes | 24 |
| Aggrecan core protein | P16112 | 2 | Yes | Swiss-Prot |
| S100 calcium-binding protein A6/calcyclin | P06703 | 3 | Yes | Swiss-Prot |
| Osteonectin/SPARC | P09486 | 18 | Yes | 17,23,24 |
| Vimentin | P08670 | 18 | Yes | 17 |
| Galectin-1 | P09382 | 6 | Yes | 17 |
| α-Crystallin | P02511 | 2 | Yes | Swiss-Prot |
| Protease and protease inhibitors | ||||
| Plasma protease (C1) inhibitor precursor | P05155 | 2 | Yes | 23 |
| Metalloproteinase inhibitor 1 precursor | P01033 | 12 | Yes | 8,24 |
| Metalloproteinase inhibitor 2 precursor | P16035 | 12 | Yes | 24 |
| Bone morphogenetic protein 1 | P13497 | 2 | Yes | Swiss-Prot |
| Ubiquitin | P62988 | 4 | Yes | 17 |
| Intracellular, contractile, and shed proteins | ||||
| Dystonin | Q5TBT2 | 12 | No | |
| Fetal myosin heavy chain | Q9Y623 | 4 | No | |
| Glyceraldehyde-3-phosphate dehydrogenase | P04406 | 3 | Yes | 25 |
| Integrin, α6 | P23229 | 2 | No | |
| Myosin binding protein C, fast type | Q14324 | 3 | No | |
| Phosphofructokinase, muscle | P08237 | 5 | No | |
| Titin | Q8WZ42 | 32 | No | |
| Triosephosphate isomerase 1 | P60174 | 6 | No | |
| Vinculin | P18206 | 6 | No | |
| Tropomyosin β | P07951 | 15 | No | |
| SH3 binding glutamic acid–rich protein | Q5T122 | 2 | No | |
| Desmin | Q549R9 | 3 | No | |
| Myosin light chain 1 | P14649 | 3 | No | |
| β-Coat protein | P53618 | 2 | No | |
| Stathmin | P16949 | 4 | No | |
| Triosephosphate isomerase | P60174 | 15 | No | |
| Ubiquitin | P62988 | 4 | No | |
| Phosphoglycerate mutase 1 | P18669 | 4 | No | |
| α-Crystallin | P02511 | 2 | No | |
| Nebulin | EAX11499 | 6 | No | |
| Myoglobin | Q8WVH6 | 3 | No |
Proteins identified using SILAC are shown with their accession numbers, number of peptides identified, and literature evidence for being secreted proteins.