FIG. 7.

C2C12 myotubes were transfected with the control nonsilencing siRNA and siRNA against FHC, MnSOD, and iNOS using the transfection reagent. The cells were used for experiments 48 h after siRNA transfection and incubated with or without recombinant adiponectin at 12 h before the experiments. ROS generations and FHC, MnSOD, and iNOS mRNA in each of the cells were determined. Each column shows the mean ± SE obtained from four samples in the presence or absence of adiponectin. *Significant difference (P < 0.05) relative to the paired control cells in the absence of adiponectin. **Significant difference (P < 0.05) relative to control siRNA-transfected cells in the presence of adiponectin. N.S., not significant relative to paired control cells in the absence of adiponectin.