FIG. 6.

The effect of hypoxia on inhibition of the insulin signaling pathway is mimicked by CoCl₂ and HIF-α expression. A: 3T3-L1 adipocytes were incubated for 16 h with CoCl₂ (200 μmol/l) and stimulated with insulin (100 nmol/l) for 5 min. B: HEK-293 cells were transfected with indicated plasmids and stimulated with insulin for 5 min. Cell lysates were analyzed by immunoblots with indicated antibodies. A representative experiment of three independent experiments performed in duplicate is shown. GFP, green fluorescence protein; IR, insulin receptor; pS6K, phospho-S6K 1; pTyr, phosphorylated tyrosine.