Skip to main content
. 2008 Dec 22;105(52):20746–20751. doi: 10.1073/pnas.0811410106

Fig. 3.

Fig. 3.

Effect of CDK and GSK-3 inhibitors on the cellular circadian rhythm. Luminescence rhythms of Bmal1-dluc cells were monitored by using ViewLux system (for GSK-3-directed inhibitors) or Tecan luminometer (for CDK-directed inhibitors) in the presence of various concentrations of compounds (10 points of 3-fold dilution series; final concentrations, 4 nM to 70 μM). (A) Luminescence profiles are indicated for each compound. Data are the representative of duplicate experiment. (B) Period parameter was obtained by the curve fitting and plotted against final concentration of the compound. Data are the mean with variation of duplicate experiment. Similar result was obtained from another experiment. Note that obtained period and phase of the basal rhythm and luminescence intensity are different between ViewLux system and Tecan luminometer, because of the setup difference that did not affect the effect of compounds (data not shown).