Fig. 4.

Effect of GSK-3β knockdown on the circadian rhythm. Bmal1-dluc cells were transfected with GSK-3β siRNA (GSK3B si1 or si2), luciferase siRNA (pGL3 si), or control siRNA (pGL2 si), and luminescence rhythms were monitored by using Tecan luminometer. After 4-d monitoring, the cells were collected for RT-qPCR analysis. (A) Endogenous GSK-3β (GSK3B) and GSK-3α (GSK3A) mRNA levels were analyzed by RT-qPCR and indicated by normalization with GAPDH. Data are the mean with variation of 2 independent experiments. (B) Luminescence profiles are indicated for each siRNA. Data are the representative of 4 independent experiments. (C) Period parameter was obtained by the curve fitting and plotted. Data from each experiment is shown by a circle, and mean period is indicated by a horizontal bar.