YC-1 stimulates HO-1 mRNA expression and promoter activity in vascular
SMCs. A, time course of HO-1 mRNA expression after the administration of YC-1
(50 μM). B, concentration-dependent increase in HO-1 mRNA after the
administration of YC-1 (5-100 μM for 24 h). C, effect of actinomycin D
(ActD; 2 μg/ml) and cycloheximide (CX; 5 μg/ml) on YC-1 (50 μM for 8
h)-mediated increase in HO-1 mRNA. D, effect of YC-1 (10-100 μM for 24 h)
on HO-1 promoter activity. HO-1 mRNA expression is expressed in arbitrary
units (a.u.). Results are means ± S.E.M. of three to five experiments.
*, statistically significant effect of YC-1.