TABLE 2.
Primers used in this study
| Primer Name | Primer Sequence | Mutation |
|---|---|---|
| pRS-Rev1-fwd | GCTTTGAGTTGGGGTAGATTATCGC | none |
| pRS-Rev1-rev | GTGTTGGTACCAAAGGAGGAGTCGGCCATTCC | none |
| rev1-1-fwd2 | TACACGAGATGATAGTTTTACATGGCaGAAAATTTT TACACTATTTGTCTTC |
rev1-1 |
| rev1-1-rev2 | GAAGACAAATAGTGTAAAAATTTTCtGCCATGTAAA ACTATCATCTCGTGTA |
rev1-1 |
| Rev1-D467A | GATTTTACCTATATCTATTGcTGcAGCTGTTTGTGTG | rev1-AA |
| E468A | AGGATAATCCC | |
| Rev1-D467A | GGGATTATCCTCACACAAACAGCTgCAgCAATAGAT | rev1-AA |
| E468A-r | ATAGGTAAAATC | |
| SC1 | GTGACGAACAGAGCTTTCGAAGCCGCCGCGGCAGC TGTAAAAAATGACATTAACAACG |
rev1-105 |
| SC1-r | CGTTGTTAATGTCATTTTTTACAGCTGCCGCGGCGG CTTCGAAAGCTCTGTTCGTCAC |
rev1-105 |
| SC2 | CTATGGAAGAACAGTTTATGAATGCAGCCGCGGCC GCAATTCGAGCAGAAGTAAGGCACG |
rev1-106 |
| SC2-r | CGTGCCTTACTTCTGCTCGAATTGCGGCCGCGGCTG CATTCATAAACTGTTCTTCCATAG |
rev1-106 |
| SC3 | TATGAATGAACTACCAACCCAAGCTGCCGCGGCAG CAAGGCACGACTTGAGAATTCAG |
rev1-107 |
| SC3-r | CTGAATTCTCAAGTCGTGCCTTGCTGCCGCGGCAGC TTGGGTTGGTAGTTCATTCATA |
rev1-107 |
| SC4 | CGGTTCAAAAAAATTTGTCAAGCCGCGGCACAAGC GGCTGCCGAAACTTTAGGTGATGGA |
rev1-108 |
| SC4-r | TCCATCACCTAAAGTTTCGGCAGCCGCTTGTGCCGC GGCTTGACAAATTTTTTTGAACCG |
rev1-108 |
| SC5 | CGAAACTTTAGGTGATGGAGGGGCGCATGCAGCAG CTGCTAAATTATTCGTGAAATATTT |
rev1-109 |
| SC5-r | AAATATTTCACGAATAATTTAGCAGCTGCTGCATGC GCCCCTCCATCACCTAAAGTTTCG |
rev1-109 |
| SC6 | GCCGCATGAAAAAGATGTTAAATTATTCGCGGCCG CGGCGGCTAAACTTTGCGATTCTAATAGAGTCCAT |
rev1-110 |
| SC6-r | ATGGACTCTATTAGAATCGCAAAGTTTAGCCGCCGC GGCCGCGAATAATTTAACATCTTTTTCATGCGGC |
rev1-110 |
| SC7 | TATTTGATTAAACTTTGCGATTCTAATGCAGCGCAT GCAGCTGCTCATTTATCAAACCTAATATCAAGGG |
rev1-111 |
| SC7-r | CCCTTGATATTAGGTTTGATAAATGAGCAGCTGCAT GCGCTGCATTAGAATCGCAAAGTTTAATCAAATA |
rev1-111 |
| SC8 | CCACTTTTAAACAGAAATAAACATGCTGCCCAGGC CGCGGCTAAACTTGACATGGACTTTGAAG |
rev1-112 |
| SC8-r | CTTCAAAGTCCATGTCAAGTTTAGCCGCGGCCTGGG CAGCATGTTTATTTCTGTTTAAAAGTGG |
rev1-112 |