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. 2009 Jan;133(1):111–127. doi: 10.1085/jgp.200810154

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© 2009 Yu et al.

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Figure 2. — Ussing chamber setup and method of correcting for junction potentials. The sign of the potential is indicated by the arrows, which show the potential of the arrow head with respect to the arrow tail. Liquid junction potentials in the agar bridge pipettes (V_L^a and V_L^b) are calculated as the potential of the solution with respect to the pipette. V_EF and V_EM are the apparent apical-basolateral potentials that are measured experimentally. (A) Setup with only a blank filter inserted between the hemichambers. The potential across the blank filter, V_F, is defined as the apical potential with respect to the basolateral side. (B) Setup with a filter on which a monolayer of cells has been cultured. The potential across the monolayer and its filter is V_M.