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. 2009 Jan;133(1):111–127. doi: 10.1085/jgp.200810154

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© 2009 Yu et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — Dose–response relationship between claudin-2 protein level and Na⁺ permeability. (A) Immunoblot showing expression of WT claudin-2 (24 kD band) induced (no doxycycline) or suppressed with varying concentrations of doxycycline. (B) Quantitation of immunoblot bands to assess claudin-2 protein abundance. (C) Permeability to Na⁺ (P_Na), as determined by NaCl dilution potential measurement.