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. 2009 Jan;133(1):43–58. doi: 10.1085/jgp.200810004

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© 2009 Zhang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 8. — Effects of the mutation at the pore entrance on the CPA-unblock rate. (A) Recovery of the current from the CPA-blocked state when the voltage was jumped from −60 to +80 mV. [CPA] = 1 mM. (B) Current recovery rate (1/τ) as a function of [CPA] in various E127/K519 combinatorial mutants. The time constant, τ, was obtained from the current-recovery process at +80 mV in those experiments shown in A.