TABLE II.

CPA Affinity (K_1/2) of E127/K519 Combinatorial Mutants

	−160 mV	−140 mV	−120 mV	−100 mV	−80 mV	−60 mV	−40 mV
E127/K519a	0.026 ± 0.001b	0.038 ± 0.001	0.061 ± 0.002	0.104 ± 0.003	0.186 ± 0.007	0.349 ± 0.015	0.581 ± 0.094
E127Q/K519	0.015 ± 0.002	0.021 ± 0.002	0.033 ± 0.003	0.057 ± 0.004	0.113 ± 0.010	0.228 ± 0.023	0.491 ± 0.058
E127/K519M	0.051 ± 0.004	0.069 ± 0.004	0.098 ± 0.004	0.141 ± 0.005	0.194 ± 0.004	0.271 ± 0.005	0.378 ± 0.007
E127/K519E	0.115 ± 0.025	0.158 ± 0.031	0.203 ± 0.034	0.277 ± 0.037	0.377 ± 0.039	0.516 ± 0.046	0.730 ± 0.059
E127Q/K519M	0.022 ± 0.002	0.031 ± 0.002	0.047 ± 0.003	0.075 ± 0.003	0.132 ± 0.006	0.240 ± 0.013	0.457 ± 0.030
E127Q/K519E	0.044 ± 0.005	0.062 ± 0.005	0.087 ± 0.006	0.123 ± 0.009	0.198 ± 0.014	0.327 ± 0.024	0.586 ± 0.052

^aThe mutations in this table are all made in the presence of the E166C mutation.

^bThe K_1/2 values are presented as mean ± SEM (in mM).