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. 2008 Jul 17;40(1):58–65. doi: 10.1165/rcmb.2007-0464OC

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Figure 4. — Treatment with N-acetyl-cysteine (NAC) significantly reduces the IL8 mRNA levels and H4 acetylation at the IL8 promoter in CFTR− cells. Cells were exposed to 5 mM NAC for 2 hour in serum-free medium. The untreated CFTR+ cell line average has been set at 1, and all other averages are normalized to that average. Significance was determined by t test. Error bars represent SEM. (A) All IL8 mRNA levels are normalized to GAPDH mRNA levels. *P < 0.05 comparing untreated CFTR+ and untreated CFTR− means using a one-tailed t test. S9/IB3: P < 0.02, 16HBE: P < 0.007, 9HTEo: P < 0.0004. **P < 0.05 for comparison of untreated CFTR− to NAC-treated CFTR− means using a one-tailed t test. S9/IB3: P < 0.05, 16HBE: P < 0.003, 9HTEo: P = 0.067; n = 3. (B) All IL8 promoter H4 acetylation levels are normalized to amount of input DNA. *P < 0.05 comparing untreated CFTR+ and untreated CFTR− means using a one-tailed t test. S9/IB3: P < 0.04, 16HBE: P < 0.0002, 9HTEo: P < 0.0002. **P < 0.05 for comparison of untreated CFTR− to NAC-treated CFTR− means using a one-tailed t test. S9/IB3: P < 0.03, 16HBE: P < 0.003, 9HTEo: P < 0.007; n = 3.

Figure 4. — Treatment with N-acetyl-cysteine (NAC) significantly reduces the IL8 mRNA levels and H4 acetylation at the IL8 promoter in CFTR− cells. Cells were exposed to 5 mM NAC for 2 hour in serum-free medium. The untreated CFTR+ cell line average has been set at 1, and all other averages are normalized to that average. Significance was determined by t test. Error bars represent SEM. (A) All IL8 mRNA levels are normalized to GAPDH mRNA levels. *P < 0.05 comparing untreated CFTR+ and untreated CFTR− means using a one-tailed t test. S9/IB3: P < 0.02, 16HBE: P < 0.007, 9HTEo: P < 0.0004. **P < 0.05 for comparison of untreated CFTR− to NAC-treated CFTR− means using a one-tailed t test. S9/IB3: P < 0.05, 16HBE: P < 0.003, 9HTEo: P = 0.067; n = 3. (B) All IL8 promoter H4 acetylation levels are normalized to amount of input DNA. *P < 0.05 comparing untreated CFTR+ and untreated CFTR− means using a one-tailed t test. S9/IB3: P < 0.04, 16HBE: P < 0.0002, 9HTEo: P < 0.0002. **P < 0.05 for comparison of untreated CFTR− to NAC-treated CFTR− means using a one-tailed t test. S9/IB3: P < 0.03, 16HBE: P < 0.003, 9HTEo: P < 0.007; n = 3.