Figure 2.

Effects of HGF on VEGF-induced EC barrier dysfunction. (A) Bovine pulmonary artery ECs (BPAEC) plated on microelectrodes were stimulated with VEGF (200 ng/ml) with or without HGF (20 ng/ml, 20 min) pretreatment, and EC permeability response to VEGF or VEGF + HGF was monitored by measurements of transendothelial electrical resistance (TER). (B) Human pulmonary EC were treated with VEGF alone, or pretreated with HGF followed by VEGF stimulation, or treated with VEGF followed by HGF addition. Immunofluorescence staining was performed using paxillin antibody. Shown are representative results of three to five independent experiments.