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Effects of HGF and VEGF on paxillin phosphorylation. HPAEC were treated with VEGF (200 ng/ml) or HGF (20 ng/ml) for the indicated periods of time. Phosphorylation of paxillin and FAK was analyzed by immunoblotting of cell lysates with a panel of phospho-specific antibodies. Equal protein loadings were verified by membrane reprobing with β-tubulin antibodies. Shown are representative results of three independent experiments.
