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. 2008 Dec 29;183(7):1203–1212. doi: 10.1083/jcb.200806068

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© 2008 Sugimura et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Localization of PARP-1 to the replicating genomic region. (A) Digoxigenin–deoxy-UTP was introduced into m5S cells to label replicating cells. PARP-1 and digoxigenin–deoxy-UTP (dig-dUTP) were visualized by immunofluorescence. DNA was counterstained with DAPI. (B and C) Localization of PARP-1–EYFP and PCNA-RFP or PARP-1–EYFP and Topo I–DsRed during S phase in COS-7 cells. After pulse labeling with BrdU, cells were fixed and BrdU was immunodetected. (D) Typical localization of PARP-1–EYFP out of S phase in COS-7 cells. (E) Localization of PARP-1–EYFP and PCNA-RFP during S phase in HeLa cells. (F) Typical localization of PARP-1–EYFP out of S phase in HeLa cells. Overlaid images and magnified views of the boxed areas are shown. n, nucleoli. Bars, 5 μm.