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. 2008 Dec 29;183(7):1213–1221. doi: 10.1083/jcb.200806048

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© 2008 Kutik et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Temperature sensitivity of Δψ generation in tam41Δ mitochondria. (A) Yeast cells were grown in YPLac at low temperature (21°C) and shifted to 30 or 37°C for 8 h in vivo before isolation of mitochondria. Δψ was assessed at 25°C by fluorescence quenching. (B) Yeast cells were grown at 21°C. Mitochondria were isolated and Δψ was assessed.