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. 2008 Dec 29;183(7):1213–1221. doi: 10.1083/jcb.200806048

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© 2008 Kutik et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — tam41Δ mitochondria are deficient in cardiolipin. (A) Lipids were extracted from isolated mitochondria and subjected to LCMS. Averaged normalized spectra from wild-type and tam41Δ mitochondria. Differential lipid profiles (log₁₀ ratio) of tam41Δ/wild-type, crd1Δ/wild-type, and taz1Δ/wild-type mitochondria. IS, internal standard (tetramyristoyl cardiolipin); PE, phosphatidylethanolamine; PI, phosphatidylinositol. (B) Analysis of cardiolipin levels in wild-type and tam41Δ mitochondria. (C) Wild-type and tam41Δ cells harboring the indicated plasmids were subjected to fivefold serial dilutions and grown at 37°C on YPD. (D) Cardiolipin levels of mitochondria from tam41Δ yeast cells harboring the indicated plasmids. Bars represent the range of two MS analyses.