Figure 4. Tetracycline treatment does not eliminate the DR response.

(A) Gel showing diagnostic PCR for the presence of the intracellular bacterium Wolbachia; (B) three strains were selected from the set of wild types for treatment with tetracycline, which was sufficient to clear Wolbachia if present. (C) Each of the three strains was then allowed at least five generations to recover on non-tetracycline-containing food before being assayed for lifespan and fecundity on different concentrations of food. Each of the three strains still exhibited a DR response after tetracycline treatment. Bars: index of lifetime fecundity±standard error of the mean; connected points: median lifespan in days. Data shown are from one of two trials in which all lifespans were run simultaneously.