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. 2008 Dec 30;3(12):e3965. doi: 10.1371/journal.pone.0003965

Figure 3. Effect of CARD-less helicases on poly-I/C induced IFNβ activation.

Figure 3

Parallel cultures of RIG-I−/− MEFs were transfected with pIFNβ-(ff)luciferase and pTK-(ren)luciferase. Some of the cultures were (or were not) transfected with plasmids expressing mda-5 (panel A; +mda-5 and ctrl) or RIG-I (panel B; +RIG-I and ctrl), along with plasmids expressing RIG-I-ΔCARD or mda-5-ΔCARD as indicated at the bottom of each panel. 24 h later, the cultures were transfected in duplicate with 5 ug of poly-I/C, as imdicated. Cell extracts were prepared after a further 20 h, and their luciferase activities were determined.