Table 1. E. bieneusi statistics with a comparison to the E. cuniculi genome.

	E. bieneusi	E. cuniculi [25]
Genome Size, Mb	61	2.9
Chromosome Number	61	11
Scaffolds	1,646	11
Scaffold N50 bp	[2,349]	NA
Contigs	[1,742]	NA
Contig N50 bp	[1,977]	NA
Assembled Mb	[3.86]	2.5
Sequence Coverage, %	[64]	86
G+C Content, %	[252]	47
Gene Models	[3,804]	2,063
Gene Density	[1/1,148 bases2]	1/1,025 bases
No. of SSU-LSU rRNA Genes	Unknown3	22
No. of 5S rRNA Genes	Unknown3	3
No. of tRNAs	[46]	46
No. and Sizes of tRNA Introns	[2 (13, 30 bp)]	2 (16, 42 bp)
No. of tRNA Synthetases	[21]	19
No. and Sizes of Splicesomal Introns	[19 (36–306 bp)]	13 (23–52 bp)
Predicted CDS4	[3,632]	1,997
Mean Intergenic Region, bp	[1272]	129
Median CDS, bp	[579]	858
Mean Size of CDS, bp	[9952]	1,077
Overlapping CDS?	Yes	Yes
No. of CDS Assigned to Functional Categories	[653 (39%)5]	884 (44%)

¹Estimation based on PFGE data.

²Values based on analysis of contigs >5 kb and their encoded genes. Bracketed values indicate values based on the survey data and not a complete genome.

³The 5S, 5.8S-SSU and LSU rRNA genes have been identified in E. bieneusi but are located on short contigs, with very short regions of sequence upstream and downstream, suggesting that these are either surrounded by sequences that are difficult to clone into E. coli or are difficult to sequence, such as highly repeated sequences. Therefore, the copy number of these genes cannot be determined at this time.

⁴CDS, coding sequences; NA, not applicable

⁵Values based on data from Table S4.