(A) Confocal images of abducens motor neurons showing punctate staining for GluR1 subunits (red) and synaptophysin (green) from each of the four groups examined: pseudoconditioned (Pseudo 2s), conditioned (Cond 2s), BDNF-treated (BDNF 2s), and BDNF with K252a-treated (BDNF + K252a 2s) preparations. (B) Confocal images abducens motor neurons showing punctate staining for GluR4 subunits (red) and synaptophysin (green) from similarly treated preparations as shown in A. In A and B, arrows indicate colocalization of AMPAR subunits with synaptophysin. (C) Quantitative analysis of GluR1, GluR4, synaptophysin, GluR1 + synaptophysin, and GluR4 + synaptophysin staining from the different treatment groups. * indicates significant differences between Pseudo 2s and Cond 2s or BDNF 2s groups; # indicates significant difference between BDNF 2s and BDNF + K252a 2s groups. Scale bar = 2 µm.