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. Author manuscript; available in PMC: 2008 Dec 23.
Published in final edited form as: J Vasc Surg. 2007 Dec;46(6):1253–1259. doi: 10.1016/j.jvs.2007.08.013

Fig. 1. Activation of phospholipase C-β (PLC-β).

Fig. 1

A, Sphingosine-1-phosphate (S-1-P, 0.1µM)–induced time-dependent activation of PLC-β, PLC-γ1, or PLC-γ2, as measured by Western blotting. Maximal activation was at 5 minutes. PLC-β activation was blocked by U7 (10nM) but not by InactiveU7 (10nM). B, A representative Western blot for PLC-β is shown. C, Bar chart shows quantification of S-1-P–induced time-dependent activation of PLC-β, PLC-γ1, or PLC-γ2, in the presence and absence of U7 and InactiveU7. Values are the mean ± SEM of the ratio of the phosphorylation of the respective protein relative to the total unphosphorylated protein (n = 6). P < .05 compared with the control. DMEM, Dulbecco minimal essential medium.