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. Author manuscript; available in PMC: 2008 Dec 23.
Published in final edited form as: J Vasc Surg. 2007 Dec;46(6):1253–1259. doi: 10.1016/j.jvs.2007.08.013

Fig E2 (online only). Oxygen free radical production: sphingosine-1-phosphate (S-1-P; 0.1µM)–induced oxygen free radical production as measured by quantitative dihydroethidium staining was significantly inhibited by U7 (10nM) but not by InactiveU7 (10nM). Representative photographs of the staining are shown.

Fig E2 (online only)

A, Dulbecco minimal essential medium (DMEM); (B) S-1-P; (C) S-1-P with InactiveU7; and (D) S-1-P with U7). E, The bar chart shows the quantification of the experiment A to D. F, The bar chart shows oxygen free radical production in response to angiotensin II and S-1-P in the presence and absence of absence of the nicotinamide dinucleotide phosphate hydrogen oxidase inhibitor, diphenyleneiodonium chloride (DPI; 10µM), as measured by quantitative dihydroethidium staining. Values are the mean ± SEM (n = 6). *P < .05; **P < 0.01 compared with control.