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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Lancet Neurol. 2008 Oct 11;7(12):1091–1098. doi: 10.1016/S1474-4422(08)70224-2

Figure 5. Effect of antibodies on the number of NMDA-receptor clusters in live neurons.

Figure 5

Representative immunoblot (A) of neuronal surface NR1 detection after incubation with control or patients’ IgG (μg/mL). Protein concentrations were normalised to concentrations of GABAA receptor subunit a2. Hippocampal neurons (B) cultured with control CSF or patients’ CSF from day 7 to day 14 in vitro (7 day treatment), or with patients’ CSF from day 7 to day 10 in vitro followed by control CSF from day 11 to day 14 in vitro (3 day treatment and 4 day recovery), then immunostained for NR1 (18–36 cells from each of three experiments). Boxed areas are shown below at higher magnification. Fewer NR1-labelled clusters (C) were found in cultures treated with patient CSF for 3 or 7 days compared with those treated with control CSF or cultures treated with patient CSF followed by 4 days recovery (18–36 cells from each of three experiments; Kruskal-Wallis non-parametric ANOVA, Dunn’s pairwise comparison, p<0.01). Incubation with patients’ CSF for 3 days or 7 days did not affect the number of PSD-95 clusters (D; 18–36 cells from each of three experiments).