TABLE 3.
Characterization of Anaplasma genotypes in different hosts and regionsa
| Species and host | Region | No. (%)b | Genotype | Sequence identity (%)c | GenBank accession no. |
|---|---|---|---|---|---|
| A. phagocytophilum | |||||
| Cattle | Western | 2 (50) | API | 95 | EU436153 |
| 1 (25) | APII | 95 | EU436154 | ||
| 1 (25) | APIII | 99 | EU436155 | ||
| Sheep | Eastern | 1 (100) | APIV | 94 | EU436156 |
| Western | 3 (100) | APII | 95 | EU436154 | |
| Mice | Eastern | 2 (100) | APV | 95 | EU436157 |
| Porcupine | Western | 1 (100) | API | 95 | EU436153 |
| Cat | Western | 1 (100) | API | 95 | EU436153 |
| A. marginale | |||||
| Cattle | Eastern | 10 (50) | AMI | 100 | EU436158 |
| 10 (50) | AMII | 99.8 | EU436159 | ||
| Western | 16 (100) | AMII | 99.8 | EU436159 | |
| A. ovis | |||||
| Sheep | Eastern | 10 (100) | AOI | 100 | EU436160 |
| Western | 10 (100) | AOI | 100 | EU436160 | |
| Goats | Eastern | 10 (100) | AOII | 99.9 | EU436161 |
| Western | 4 (100) | AOI | 100 | EU436160 |
a
The 16S rRNA and msp4 gene amplicons were cloned and sequenced for the characterization of Anaplasma genotypes. All A. phagocytophilum 16S rRNA gene-positive fragments were used for genotype characterization in A. phagocytophilum, and randomly selected msp4 sequences were used for analysis of A. marginale and A. ovis strains.
b
Number of strains with this genotype (percentage of total samples analyzed).
c
Percent sequence identity at the nucleotide level with respect to the reference sequence (DQ029028 for A. phagocytophilum 16S rRNA genes and AY702920 and AY702923 for A. marginale and A. ovis msp4, respectively).